Design of an Experimental Study for the Simultaneous Determination of Cefepime, Piperacillin and Tazobactam Using Micellar Organic Solvent-Free HPLC

Application of Sustainable analytical chemistry concepts has become crucial in order to remove the environmentally harmful impacts originating from routine use techniques. Here, a new LC method is developed and its parameters are analyzed, depending on mixed micellar mobile phase. This was primarily aimed at getting rid organic solvents conventional analyses. Combinations tazobactam (TZB) with piperacillin (PPC) or cefepime (CFM) commonly used as effective antimicrobial therapies, especially for resistant strains. Therefore, three drugs were separated quantified using an solvent-free The phase comprised 15 mM Brij-35 38 SDS, adjusted pH 3.5. Separation performed by HPLC monolithic RP-C18 column Chromolith® Performance RP-18e (100 mm × 4.6 mm) rate 1 mL per minute flow conjunction measurement wavelength 210 nm. found valid applicable accordance precision, accuracy within ranges 5–100 µg mL?1 PPC CFM 0.625–12.5 TZB. quality-by-design technique analyze effect modifying ratios separation efficiency conclude their behavior. Finally, suggested approach assessed applying green procedure index against greenest published methodology show superiority.